OPTIMIZATION OF PHYTOESTROGENS EXTRACTION FROM SOY GERM USING RESPONSE SURFACE METHODOLOGY96 views
Keywords:Soy germ; Phytoestrogens; RSM.
Phytoestrogens are phytochemicals with antioxidant activities and potential health benefits. Their contents in soy germ is the highest compared to that in parts of soy seed. Response surface methodology (RSM) using Box Behnken Design of four factors was employed to optimize the extraction conditions for phytoestrogens. The Box Behnken Design with five replicates at central point was applied. The four independent variables investigated in this experiment were extraction time (X1) 60-120 minutes, solvent/solid ratio (X2) 8-12, extraction pH (X3) 8-10, ethanol concentration (X4) 50-70%. The high coefficient values indicated that the variables were fitted to the regression for the total phytoestrogens (R2 = 0.9887). Optimum conditions for maximizing total crude phytoestrogen content were 90 minutes for the extraction time, 12/1 for the extraction ratio, 9 for the extraction pH, and 65% for the ethanol concentration.
. J.-A. Kim, S.-B. Hong, W.-S. Jung, C.-Y. Yu, K.-H. Ma, J.-G. Gwag, and I.-M. Chung, "Comparison of isoflavones composition in seed, embryo, cotyledon and seed coat of cooked-with-rice and vegetable soybean (Glycine max L.) varieties". Food Chemistry. Vol. 102. pp 738-744, (2007).
. H. Wang and P. A. Murphy, "Isoflavone Content in Commercial Soybean Foods". Journal of Agricultural and Food Chemistry. Vol. 42. pp 1666-1673, (1994)
. J. Chen, G. Tang, J. Zhou, W. Liu, and Y. Bi, "The characterization of soybean germ oil and the antioxidative activity of its phytosterols". The Royal Society of Chemistry Advances. Vol. 9. pp 40109-40117, (2019).
. S. M. Bae, C. H. Jang, J. H. Kim, H. A. Lim, J. R. Kim, J. H. Kim, and J. S. Kim, "Optimization of Isoflavone Extraction from Soy Germ". Journal of Food Science and Nutrition. Vol. 10. pp 290-293, (2005).
. M. C. Carrão-Panizzi, S. P. de Goes Favoni, and A. Kikuchi, "Extraction Time for Soybean Isoflavone Determination". Brazilian Archives of Biology and Technology. Vol. 45. pp 515-518, (2002).
. M. Wang, "An Effective and Green Method for the Extraction and Purification of Aglycone Isoflavones From Soybean". Food Science and Biotechnology. Vol. 22. pp 705-712, (2013).
. S. Y. Cho, Y. N. Lee, and H. J. Park, "Optimization of ethanol extraction and further purification of isoflavones from soybean sprout cotyledon". Food Chemistry. Vol. 117. pp 312-317, (2009).
. X.-L. Yu and Y. He, "Application of Box-Behnken designs in parameters optimization of differential pulse anodic stripping voltammetry for lead(II) determination in two electrolytes". Scientific Reports. Vol. 7. pp 2789, (2017).
. D. T. H. Vien and D. T. Trang, "Research on extraction of isoflavones from soybean germ". Vietnam Journal of Science and Technology. Vol. 54. pp 61-68, (2018).