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APPLICATION OF BOX-BEHNKEN DESIGN WITH RESPONSE SURFACE METHODOLOGY FOR OPTIMIZING SYNTHESIS CONDITIONS OF OBIDOXIME CHLORIDE

Toan Ngoc Vu, Tri Minh Nguyen, Trinh Kieu Truong, Minh Quang Luu

Abstract


Obidoxime chloride is one of antidotes for organophosphorus poisoning. The process for synthesis obidoxime chloride was started from pyridine-4-aldoxime. A combination three factor of molar ratio, temperature and reaction time was used to synthesize to obidoxime chloride. This showed that the combined process could be an effective method of synthezing obidoxime chloride. This combined process was successfully modeled and optimized using Box-Benhnken design with response surface methodology (RSM). The effects of the molar ratio, temperature and reaction time were investigated. Analysis of variance indicated that the prosed quadratic model successfully interpreted the experimental data with coefficients of determination of R2 = 0.9205. Through this model, we could predict the synthesis efficiency under different conditions. Based this model, the optimal conditions for the synthesis of obidoxime chloride were found to be a molar ratio of 1.8283, temperature of 60 oC and reaction time of 30 minitues. The predicted synthesis yield obtained from RSM under the optimal conditions was 81.31 %. A confirmation test of the optimal conditions verified the validity of the model, yielding an synthesis efficiency of 80.83 %.

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References


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