Selection of an enzyme-linked immunoassay method to detect dioxin contamination in the environment
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https://doi.org/10.54939/1859-1043.j.mst.VITTEP.2022.100-106Keywords:
Competitive ELISA; Sandwich ELISA; Dioxin.Abstract
Enzyme-linked immunosorbent assay (ELISA) is one of the methods being studied for screening for dioxin contamination because of the advantages of rapid, low cost and easy in-field implementation. The two methods of most interest are sandwich ELISA and indirect competitive ELISA with their own advantages. Therefore, it is necessary to study and investigate the dioxin detection ability of ELISA methods. This research initially evaluated the ability to detect dioxin of two ELISA methods with some commercially available anti-dioxin antibodies. The results showed that the anti-dioxin CABT-L4232 antibody had good binding ability to dioxins and hapten. Initially, the study showed that the indirect competitive ELISA method was capable of detecting dioxins at a concentration of 250 ppt, while the sandwich ELISA method was capable of detecting dioxins at a concentration of 1000 ppt in the analytical solution. Therefore, the indirect competitive ELISA method was chosen to continue to be optimal for dioxin analysis.
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