AN EXPERIMENTAL STUDY ON DETECTION OF DIOXIN USING SANDWICH ELISA

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Authors

  • Tran Thi Thanh Quynh (Corresponding Author) Institute of New Technology, Academy of Military Science and Technology

Keywords:

Detect dioxin; Aryl hydrocarbon receptor; Sandwich ELISA; 2,3,7,8-Tetrachlorodibenzo-p-dioxin.

Abstract

Dioxin is a group of highly toxic chemical compounds including 210 congeners, causing serious diseases on human health such as cancer, genetic diseases, birth defects and others. In particular, 2,3,7,8 -Tetrachlorodibenzo-ρ-dioxin (TCDD) was determined to have the highest toxic coefficient. In this study, we used the Sandwich ELISA method based on Aryl hydrocarbon receptor and monoclonal antibody to detect TCDD in 1% DMSO. The initial results showed that the immunoassay was capable of detecting TCDD with a detection limit of 1 pg/ml (1 ppt), a sensitivity of 97,14% and a specificity of 91,67%.

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Published

10-04-2020

How to Cite

Quỳnh. “AN EXPERIMENTAL STUDY ON DETECTION OF DIOXIN USING SANDWICH ELISA”. Journal of Military Science and Technology, no. 66, Apr. 2020, pp. 123-30, https://en.jmst.info/index.php/jmst/article/view/231.

Issue

No. 66 (2020)

Section

Research Articles