Antibacterial and antioxidant lemongrass essential oil Pickering emulsion stabilized by cellulose nanocrystals

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Authors

  • Nguyen Thi Hai Yen Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)
  • Ly Boi Tuyen Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)
  • Bui Thi Anh Bup Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)
  • Le Anh Kien Institute for Tropical Technology and Environmental Protection, Academy of Military Science and Technology
  • Le Thi Kim Phung (Corresponding Author) Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)

DOI:

https://doi.org/10.54939/1859-1043.j.mst.89.2023.87-93

Keywords:

Lemongrass essential oil; Cellulose nanocrystals; Pickering emulsion; Antimicrobial; Antioxidant.

Abstract

An effective antibacterial system was developed by using cellulose nanocrystals (CNC) to stabilize lemongrass essential oil Pickering emulsion (PE-LEO) through ultrasonication technology. The factors affecting the formation and stability of PE-LEO were studied, such as ultrasonication times, CNC concentrations, lemongrass essential oil (LEO) concentrations. By size and zeta index, the most suitable sample was 8 ultrasonication times, 0.8% CNC, 15% LEO. The antibacterial and anti-fungal performance of PE-LEO was investigated by determining the minimal inhibitory concentrations (MIC). The results showed that for gram-positive bacteria (E.faecalis, S.aureus, MRSA), the MIC of PE-LEO was much higher than LEO, the opposite was true for gram-negative bacteria (E. coli) and fungi. Based on the concentrations of LEO, with IC50 of PE-LEO is 0.30% vLEO/v, which is significantly lower than that of LEO (0.99%). The CNC-stabilized PE-LEO exhibited higher antioxidation activity at equivalent LEO concentrations. The fabricated CNC based Pickering emulsions provide a promising alternative for the delivery of antimicrobial essential oils in the food industries.

References

[1]. Gao, C., et al., "Essential oil composition and antimicrobial activity of Sphallerocarpus gracilis seeds against selected food-related bacteria". Food Control. 22(3): p. 517-522, (2011). DOI: https://doi.org/10.1016/j.foodcont.2010.09.038

[2]. Chouhan, S., K. Sharma, and S.J.M. Guleria, "Antimicrobial activity of some essential oils—present status and future perspectives". 4(3): p. 58, (2017). DOI: https://doi.org/10.3390/medicines4030058

[3]. Cossu, A., et al., "Antifungal activity against Candida albicans of starch Pickering emulsion with thymol or amphotericin B in suspension and calcium alginate films". International Journal of Pharmaceutics. 493(1): p. 233-242, (2015). DOI: https://doi.org/10.1016/j.ijpharm.2015.07.065

[4]. Souza, A.G., et al., "Starch-based films enriched with nanocellulose-stabilized Pickering emulsions containing different essential oils for possible applications in food packaging". 27: p. 100615, (2021). DOI: https://doi.org/10.1016/j.fpsl.2020.100615

[5]. Zhou, X., et al., "Development and characterization of bilayer films based on pea starch/polylactic acid and use in the cherry tomatoes packaging". Carbohydrate Polymers. 222: p. 114912, (2019). DOI: https://doi.org/10.1016/j.carbpol.2019.05.042

[6]. Mikulcová, V., R. Bordes, and V. Kašpárková, "On the preparation and antibacterial activity of emulsions stabilized with nanocellulose particles". Food Hydrocolloids. 61: p. 780-792, (2016). DOI: https://doi.org/10.1016/j.foodhyd.2016.06.031

[7]. Vörös-Horváth, B., "Application of Pickering emulsions in pharmaceutical technology for formulation of water insoluble drugs and essential oils": University of Pécs, Faculty of Pharmacy Institute of Pharmaceutical Technology and Biopharmacy. p. 3, (2021).

[8]. Bao, X., J. Wu, and G.J.P.i.N.S.M.I. Ma, "Sprayed Pickering emulsion with high antibacterial activity for wound healing". 30(5): p. 669-676, (2020). DOI: https://doi.org/10.1016/j.pnsc.2020.08.001

[9]. Horváth, B., et al., "Preparation and in vitro diffusion study of essential oil Pickering emulsions stabilized by silica nanoparticles". 33(6): p. 385-396, (2018). DOI: https://doi.org/10.1002/ffj.3463

[10]. Seo, S.-M., et al., "Development of cellulose nanocrystal-stabilized Pickering emulsions of massoia and nutmeg essential oils for the control of Aedes albopictus". 11(1): p. 1-12, (2021). DOI: https://doi.org/10.1038/s41598-021-91442-6

[11]. Shin, J., et al., "Biological activity of thyme white essential oil stabilized by cellulose nanocrystals". 9(12): p. 799, (2019). DOI: https://doi.org/10.3390/biom9120799

[12]. Naik, M.I., et al., "Antibacterial activity of lemongrass (Cymbopogon citratus) oil against some selected pathogenic bacterias". Asian Pacific Journal of Tropical Medicine. 3(7): p. 535-538, (2010). DOI: https://doi.org/10.1016/S1995-7645(10)60129-0

[13]. Haque, A.N.M.A., R. Remadevi, and M. Naebe, "Lemongrass (Cymbopogon): a review on its structure, properties, applications and recent developments". Cellulose. 25(10): p. 5455-5477, (2018). DOI: https://doi.org/10.1007/s10570-018-1965-2

[14]. Morales-Narváez, E., et al., "Nanopaper as an Optical Sensing Platform". ACS Nano. 9(7): p. 7296-7305, (2015). DOI: https://doi.org/10.1021/acsnano.5b03097

[15]. Kim, M., et al., "Monolithic Chiral Nematic Organization of Cellulose Nanocrystals under Capillary Confinement". ACS Nano. 15(12): p. 19418-19429, (2021). DOI: https://doi.org/10.1021/acsnano.1c05988

[16]. Vo, N., et al., "Recovery of Rice Straw Cellulose on Pilot Scale for Fabrication of Aerogel for Oil/Water Separation". 97: p. 73-78, (2022).

[17]. Linke, C., S.J.C.r.i.f.s. Drusch, and nutrition, "Pickering emulsions in foods-opportunities and limitations". 58(12): p. 1971-1985, (2018). DOI: https://doi.org/10.1080/10408398.2017.1290578

[18]. Freitas, C. and R.H.J.I.J.o.P. Müller, "Effect of light and temperature on zeta potential and physical stability in solid lipid nanoparticle (SLN) dispersions". 168: p. 221-229, (1998). DOI: https://doi.org/10.1016/S0378-5173(98)00092-1

[19]. Yu, H., et al., "Cellulose nanocrystals based clove oil Pickering emulsion for enhanced antibacterial activity". International Journal of Biological Macromolecules. 170: p. 24-32, (2021). DOI: https://doi.org/10.1016/j.ijbiomac.2020.12.027

[20]. He, Y., et al., "Factors that Affect Pickering Emulsions Stabilized by Graphene Oxide". ACS Applied Materials & Interfaces. 5(11): p. 4843-4855, (2013). DOI: https://doi.org/10.1021/am400582n

[21]. Zhou, Y., et al., "Preparation and antimicrobial activity of oregano essential oil Pickering emulsion stabilized by cellulose nanocrystals". International Journal of Biological Macromolecules. 112: p. 7-13, (2018). DOI: https://doi.org/10.1016/j.ijbiomac.2018.01.102

[22]. Dickinson, E.J.F.h., "Hydrocolloids as emulsifiers and emulsion stabilizers". 23(6): p. 1473-1482, (2009). DOI: https://doi.org/10.1016/j.foodhyd.2008.08.005

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Published

25-08-2023

How to Cite

Nguyen Thi, H. Y., Ly Boi Tuyen, Bui Thi Anh Bup, Le Anh Kien, and Le Thi Kim Phung. “Antibacterial and Antioxidant Lemongrass Essential Oil Pickering Emulsion Stabilized by Cellulose Nanocrystals”. Journal of Military Science and Technology, vol. 89, no. 89, Aug. 2023, pp. 87-93, doi:10.54939/1859-1043.j.mst.89.2023.87-93.

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