Determining the conditions for obtaining Bacillus subtilis biomass to create Synbiotic-Plus
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https://doi.org/10.54939/1859-1043.j.mst.FEE.2022.253-259Keywords:
Bacillus subtilis; Synbiotic; Probiotic; Prebiotic.Abstract
Synbiotics are food supplements that protect the health, they are made up of two main ingredients, fiber (prebiotic) and probiotics (probiotic). Synbiotics have the function of beneficial bacteria, balancing the microbiome in the gut. In Vietnam, Bacillus subtilis is widely used in probiotic production. Bacillus subtilis is of interest because of its spore-forming ability. Spores can germinate and grow under anaerobic conditions in the intestinal tract, thus maintaining a population of beneficial bacteria in the gut. The purpose of this study is to evaluate the influence of medium factors on the growth and development of the strain and determine some properties of the strain, thereby making a premise for fermentation to produce Synbiotic-Plus. The results determined the conditions for culturing Bacillus subtilis strains: the optimal media for the growth and development of B. subtilis strain was DSM, pH 7, 37 °C, speed 200 rpm, 5 days of incubation. Spore formation efficiency was 85,57%. The strain B. subtilis exhibits the properties of the probiotic strain such as the ability to produce extracellular enzymes (cellulase, amylase, protease), antibacterial ability (E. coli, Salmonella).
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